|
|
|
|
|
| Ohmori, Takashi; Tamahara, Satoshi; Thi, Pham Ngoc; Tomihari, Mizuki; Shimada, Terumasa; Matsuki, Naoaki; Ono, Kenichiro. |
| Interleukin-12 (IL-12) is one of the most important factors for the host’s protective immune response against protozoan infection. To evaluate the effect of bioactive form of IL-12(IL-12p70), expression of IFN-AmRNA and IL-4 mRNA in splenic CD4 positive T cells, anti-parasite delayed type hypersensitivity (DTH), and serum and splenic IL-12p70 concentration were examined in Babesia microti and B. rodhaini infected mice. The expression of IFN-AmRNA as a marker of Th1 cell differentiation was detected in splenic CD4 positive T cells in B. microti infected mice. Activation of DTH response as a marker of cell-mediated immune response was also observed from 2 days after the infection with B. microti, while no response was detected in B. rodhaini infected mice.... |
|
Palavras-chave: Babesia microti; Babesia rodhaini; Cell-mediated immunity; Early phase of infection; IL-12p70; Th1 cell differentiation. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/150 |
| |
|
| |
|
| |
|
|
| Horn, Karin; Ono, Kenichiro; Heydorn, Alfred Otto. |
| This paper describes a standard method for in vitro excystation of sporocysts of S.capracanis, S.hircicanis, S.ovicanis and S.arieticanis. A standard pretreatment was performed by 20 minutes preincubation of sporocysts in aqueous sodium hypochlorite (NaOCl) solution of 6% or 8% at room temperature. Then, sporocysts were washed 5 times in distilled water and were incubated for one hour at 39OC in excystation fluid consisting of RPMI 1640 medium, 10% PCS and 15% bovine bile. Additional sonication of pretreated sporocysts increased excystation rates as compared to not sonicated controls. Excystation rates for 1-9 months old sporocysts were 77% for S.capracanis, 77% for S.hircicanis, 72% for S.arieticanis and 92% for S.ovicanis. Sporozoite suspension as... |
|
Palavras-chave: Cryopreservation; In vitro excystation; Sarcocystis spp.; Sporozoites. |
| Ano: 1991 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/154 |
| |
|
|
| Fujii, Yasuhiro; Maki, Yoshiyuki; Ito, Masanari; Saito, Atsushi; Igarashi, Ikuo; Ono, Kenichiro; Itoh, Kikuji; Suzuki, Naoyoshi. |
| Large numbers of cyclophosphamide- or carrageenan-pretreated immunosuppressed adult ddY mice died within 10 days after inoculation with several species of opportunistic bacteria, including P. aeruginosa, K. pneumoniae, E. coli, S. aureus and methicillin resistant S. aureus. When immunosuppressed mice were administered bacteria in combination with two 100 μg/mouse intramuscular doses of a newly synthesized peptide, Obiopeptide-1 (OP-1), survival rates increased significantly. At 24 and 48 hours after intraperitoneal inoculation with P. aeruginosa, counts of viable organisms from the livers, spleens, lungs, hearts, and kidneys of mice that were administered cyclophosphamide in combination with OP-1 were significantly lower than counts from mice that were... |
|
Palavras-chave: Immunoregulator; Synthesized peptide; Immunosuppression; Cyclophosphamide; Carrageenan; Ooiopeptide. |
| Ano: 1992 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/166 |
| |
|
| |
|
| |
|
|
| Hashiguchi-Kato, Rie; Thi, Pham Ngoc; Ohmori, Takashi; Tamahara, Satoshi; Katayama, Shinsuke; Shimada, Terumasa; Matsuki, Naoaki; Ono, Kenichiro. |
| Stimulatory factors for interleukin 12 (IL-12) production associated with Babesia microti and Babesia rodhaini infected erythrocytes were examined using an in vitro assay system established, since a remarkable increase of serum IL-12 concentration and differentiation of helper T cell (Th cell) into helper T cell type 1 (Th1 cell) was observed in early phase of infection with Babesia spp. in mice. To investigate direct stimulating activity of infected erythrocytes for IL-12 production, intact splenic macrophages were co-cultured with them and examined the expression of IL-12 mRNA by reverse transcription-polymerase chain reaction (RT-PCR). Both B. microti and B. rodhaini infected erythrocytes elicited IL-12 mRNA expression in co-cultured macrophages. Since... |
|
Palavras-chave: Babesi microti and Babesia rodhaini infected erythrocyte; IL-12 Production; Mice; Splenic Macrophage; Stimulatory factor. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/151 |
| |
|
| |
|
|
|
